A direct method of gene transfer which is suitable for plants, in this cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA is ?

Options

A. Microinjection
B. Biolistics or Gene gun
C. Disarmed pathogen
D. Electroporation

Answer

To answer the question regarding the direct method of gene transfer suitable for plants, where cells are bombarded with high-velocity micro-particles of gold or tungsten coated with DNA, we can follow these steps:

### Step-by-Step Solution:

1. **Understand the Question**: The question is asking for a specific method of gene transfer that is direct and suitable for plants. It mentions the use of high-velocity micro-particles of gold or tungsten coated with DNA.

2. **Identify the Options**: The options provided are:
- Microinjection
- Biolistics or Gene Gun
- Disarmed Pathogen
- Electroporation

3. **Analyze Each Option**:
- **Microinjection**: This method involves injecting DNA directly into the nucleus of a cell using a micropipette. It is primarily used for animal cells, not plants.
- **Biolistics or Gene Gun**: This method involves bombarding plant cells with high-velocity micro-particles (like gold or tungsten) that are coated with DNA. This is a well-known technique for gene transfer in plants.
- **Disarmed Pathogen**: This method involves using a modified pathogen to transfer genes into a host cell, but it does not specifically involve the bombardment of micro-particles.
- **Electroporation**: This technique uses an electrical field to increase the permeability of the cell membrane, allowing DNA to enter. It is not specific to the method described in the question.

4. **Select the Correct Answer**: Based on the analysis, the method that fits the description of bombarding cells with high-velocity micro-particles coated with DNA is the **Biolistics or Gene Gun**.

5. **Conclusion**: Therefore, the correct answer to the question is **Biolistics or Gene Gun**.