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With respect to DNA fragmentation ,State...

With respect to DNA fragmentation ,Statement (A) Gel electrophoresis and elution are two important processes. Statement (B) After staining with ethidium bromide it has to be exposed to UV light.

A

Only A is correct.

B

Both A and B are correct.

C

Only B is correct.

D

Only A is correct and B is incorrect

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Diagram shows a typical agarose gel showing migration of DNA fragments . a. Which of the bands has the largest and smallest DNA fragments ? b. How can you make fragments of DNA for electrophoresis ? c. Explain separation of DNA fragments using electrophoresis. d. Point out a method to visualize the separated DNA fragments after electrophoresis .

Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.

Which of the following statements are correct ? (i) Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome site, but between the same two bases on the opposite strands. (ii) Hind ll always cuts DNA molecules at a particular point by reconginisng a specific sequence of six base pairs. (iii) Separated DNA fragments cannot be visualised without staining on an agarose gel electrophoresis. (iv) 'Ori' is the sequence responsible for controlling the copy number. (v) DNA is a positively charged molecule.

Match the terms given in column I with their definitions in column II and select the correct answer from codes given below. {:(Column I,Column II),(A."Transformation",(i)"Sequences cut by restriction enzymes"),(B."Recognition site",(ii)"Process by which DNA fragments are separated based on their size"),(C."Gel electrophoresis",(iii)"Plasmid DNA that has incorporated human DNA"),(D."Recombinant DNA",(iv)"Process by which bacteria take up pieces of DNA from the environment"):}

Match the words in box 'A' with the most accurate answers from box 'B' {:(" "A," "B),("i. Lysozyme","a. T-DNA"),("ii. Agrobacterium tumifaciens","b. DNA stain"),("iii. Gel electro-phoresis","c. Escherichia coli"),("iv. Ethidium bromide","d . Restriction enzymes"),("v. Eco Ri","e. Bacterial cell breakage"),("vi. Molecular scissors","f. Separation of DNA fragments"):}

SARAS PUBLICATION-MODEL QUESTION PAPER 5-EXERCISE
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  2. A scrubber in the exhaust of a chemical industrial plant removes:

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  4. The pioneer species in Xerarch and hydrarch succession are respectivel...

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  9. Identify the wrong statement:

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  10. Continued self-pollination results in:

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  11. Which vector can clone only a small fragment of DNA?

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  12. Seeds without fertilisation is obtained from:

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  16. The ancestors of salamanders are:

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  18. The nitrogen base found only DNA is also called:

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  20. Which of the following statement is correct?

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