The DNA fragments separated on an agaros...

The DNA fragments separated on an agarose get can be visualised after staining with:

Acetocarmine

Aniline blue

Ethidium bromide

Bromophenol blue

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DNA fragments are:

Which of the following statements are correct ? (i) Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome site, but between the same two bases on the opposite strands. (ii) Hind ll always cuts DNA molecules at a particular point by reconginisng a specific sequence of six base pairs. (iii) Separated DNA fragments cannot be visualised without staining on an agarose gel electrophoresis. (iv) 'Ori' is the sequence responsible for controlling the copy number. (v) DNA is a positively charged molecule.

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Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.

Ortho and para-nitro phenol can be separated by

Diagram shows a typical agarose gel showing migration of DNA fragments . a. Which of the bands has the largest and smallest DNA fragments ? b. How can you make fragments of DNA for electrophoresis ? c. Explain separation of DNA fragments using electrophoresis. d. Point out a method to visualize the separated DNA fragments after electrophoresis .

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