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DNA fragments are:...

DNA fragments are:

A

Negatively charged

B

Neutral

C

Either positively or negatively charged depending on their size

D

Positively charged

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During genetic engineering DNA fragments are produced by cutting DNA with endonucleases. These fragments are further used for Producing recombinant DNA. Explain the technique and principle that help to sepa-rate these DNA fragments .

Match the terms given in column I with their definitions in column II and select the correct answer from codes given below. {:(Column I,Column II),(A."Transformation",(i)"Sequences cut by restriction enzymes"),(B."Recognition site",(ii)"Process by which DNA fragments are separated based on their size"),(C."Gel electrophoresis",(iii)"Plasmid DNA that has incorporated human DNA"),(D."Recombinant DNA",(iv)"Process by which bacteria take up pieces of DNA from the environment"):}

Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.

Diagram shows a typical agarose gel showing migration of DNA fragments . a. Which of the bands has the largest and smallest DNA fragments ? b. How can you make fragments of DNA for electrophoresis ? c. Explain separation of DNA fragments using electrophoresis. d. Point out a method to visualize the separated DNA fragments after electrophoresis .

How are the DNA fragments separated through gel electrophoresis ex-tracted ?

Restriction endonucleases are used to join the DNA fragments having sticky ends.

What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

The DNA fragments separated on an agarose get can be visualised after staining with:

SARAS PUBLICATION-GENETICS AND EVOLUTION-EXAMPLE
  1. The two polypeptides of human insulin are linked together by:

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  2. Which of the following is a restriction endonuclease?

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  3. A cell at telophase stage is a student in a plant brought from the fie...

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  4. Taylor conducted the experiment to prove semiconservative replication ...

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  5. The mechanism that causes a gene to move from one linkage group to ano...

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  6. Which of the following rRNAs acts as structural RNA as well as ribozym...

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  7. Which of the following restriction enzymes produces blunt ends?

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  8. If a colour - blind man marries a woman who is homozygous for normal c...

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  9. Genetic drift leads to......

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  10. In Hardy - Weinberg equation, the frequency of heterozygous individual...

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  11. A molecule that can act as a genetic material must fulfill the traits ...

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  12. Match the satge of meiosis in Column - I to their characteristic featu...

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  13. The DNA fragments separated on an agarose get can be visualised after ...

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  14. The final proof for DNA as the genetic material came from the experime...

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  15. Plants which produce characteristic pneumatophores and show vivipary b...

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  16. DNA fragments are:

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  17. Which of the following RNAs should be most abundant in animal cell?

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  18. What is the criterion for DNA fragments movement on agarose gel during...

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  19. DNA replication in bacteria occurs:

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  20. Anaphase promoting complex APC is a protein degraddation macchinery ne...

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