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For transformation, micro -particles coa...

For transformation, micro -particles coated with DNA to be bombarded with gene gun are made up of

A

Silver or Platinum

B

Platinum or Zinc

C

Silicon or Platinum

D

Gold or Tungsten

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For transformation, micro-particles coated with DNA to be bombarded from gene gun are made up of (a) silver or platium (b) platinum or zinc (c) silicon or platinum (d) gold or tungsten

In biolistic method of gene trasfer, the microparticles coated with foreign DNA are bombarded into target cells at a very high velocity. These microparticles are made up of 1) silver or tungsten 2) arsenic or silver 3) gold or tungsten 4) none of these

The seed coat of ground nut is made up of:

Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.

A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reason could be (a) Human gene may have intron which bacteria cannot process (b) Amino acid codons for humans and bacteria are different (c) Human protein is formed but degraded by bacteria (d) all of the above

(i) The RNA polymerase, opens up the DNA for replication (ii) There more than one tRNA for most amino acids. (iii) The split gene feature of eukaryote genes is absent in prokaryotes. (iv) The X chromosome is the smallest chromosome.

If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred to E.coli cells, the host cells become transformed into ampicillin resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grown and the untransformed recipient cells will die. The ampicillin resistant gene in this case is called as 1) selectable marker 2) recombinant protein 3) cloning site 4) chemical scalpels

Transgenic animals are animals that have had their DNA manipulated to possess and express an extra gene. Comment on some of the areas where these transformed organisms are used.

The different steps of recombinant DNA technology are given below randomly. (i) Isolation of the DNA fragments or genes to be cloned (ii) Introduction of the recombinant DNA into a suitable cell (usually E. coil) called host (transformation) (iii) Multiplication/expression of the introduced gene in the host (iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment (v) Insertion of the isolated gene in a suitable plasmid vector Which of the following represents the correct sequences of steps ?

SARAS PUBLICATION-BIOTECHNOLOGY AND ITS APPLICATIONS-EXAMPLE
  1. Which one is the most abundant protein in the animal world?

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  2. Even in absence of pollinating agents seed setting is assured in

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  3. For transformation, micro -particles coated with DNA to be bombarded w...

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  4. Which of the following statements is not true of two genes that show 5...

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  5. Which of the following Bt crops is being grown in India by the farmers...

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  6. The colonies of recombinant bacteria appear white in contrast to blue ...

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  7. Kyoto Protocol was endorsed at

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  8. If both parents are carriers for thalassemia , which is an autosomal r...

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  9. Uridine,present only in RNA is a

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  10. Satellite RNAs are present in some

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  11. Which of the following has maximum genetic diversity in india ?

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  12. In an inducible operon, the genes are

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  13. Which one of the following is hallucinogenic drug?

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  14. Which one of the following statement is correct

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  15. In S phase , Amount of DNA in each cell?

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  16. Which of the following shows coiled RNA strand and capsomeres?

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  17. Which one of the following is wrongly matched?

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  18. The first human hormone produced by recombinant DNA technology is

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  19. Select the correct option: Direction of RNA Synthesis & Direction of r...

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  20. The structures that are formed by stacking of organised flattened memb...

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