Mention the number of primers required in each cycle of PCR. Writen the role of primers and DNA polymerase in PCR. Name the source organism of DNA polymerase used in PCR.

What is the significance of thermostable DNA polymerase during PCR ? From where is this DNA polymerase extracted ?

Which of the following statements are true regarding DNA polymerase used in PCR ?

Name the various types of prokaryotic DNA polymerase. State their role in replication process.

A policeman finds a very small piece of body tissue from the site of a crime and takes it to the forensic department. a. By which technique will they amplify the DNA collected from the tissue sample? b. Mention in a sequence, the three steps involved in each cycle of this technique. c. What is the role of thermostable DNA polymerase in this technique?

Assertion (A) : DNA polymerase alpha synthesizes primers. Reason (R) : DNA polymerase requires a free 3' OH to initiate DNA synthesis.

Identify the correct statements from the below I. Thermus aquatics is a bacterium which can tolerate high temperatures. (II) The first DNA polymerase enzyme was isolated from thermus aquatics. (III) T. aquatics used in polymerase chain reaction (PCR) for DNA amplification. (IV) Cyanobacteria are photosynthetic blue green algae.