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Ribosomes And Inclusion Bodies

Structural Of Bacteria|Gram Positive And Negative Bacteria|Difference Between Gram Positive And Gram Negative|Ribosomes And Inclusion Bodies

Given below are some step for slicing foreign DNA into a plasmid and then inserting the plasmid into a bacterium. (i) Transform bacteria with recombinant (ii) Cut the plasmid DNA using restriction enzyme . (iii) Extract plasmid DNA to - DNA fragments DNA (iv) Bind the plasmid DNA to non-plasmid DNA fragments . (v) Use ligase to seal plasmid DNA to non- plasmid DNA Arrange the above steps in the right sequence and select the CORRECT option

I. Transform bacteria with recombinant DNA molecule. II. Cut the plasmid DNA using restriction enzymes. III. Extract plasmid DNA from bacteria cells. IV. Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments. V. Use ligate to seal plasmid DNA to non-plasmid DNA. From the given list, which of the following is the most logical sequence of steps for splicing foregain DNA into a plasmid and inserting the plasmid into a bacterium ?

A plasmid is a :

Plasmids

DNA of plasmid is