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Guard cells are responsible for openin...

Guard cells are responsible for opening and closing of the stomata

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Opening and closing of stomata takes place due to changes in turgor of guard cell. The turgor changes in the guard cells are due to entry and exit of water into and out of the guard cells. During the day, water from the subsidiary cells enter the guard cells making it fully turgid causing the stomata to open. During night time, water from guard cells enters the subsidiary cells makes the guard cells flaccid causing the stomata closes.
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