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Describe the tools of recombinant DNA te...

Describe the tools of recombinant DNA technology.

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Techniques of Genetic Engineering - Basic Requirements:
Important discoveries that led to the stepping stone of rDNA technology were
(i) Presence of plasmid in bacteria that can undergo replication independently along with chromosomal DNA.
(ii) Restriction enzymes cuts or break DNA at specific sites and are also called as molecular scissors.
(iii) DNA ligases are the enzymes which help in ligating (joining) the broken DNA fragments.
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The flow chart given below represents the process of recombinant DNA technology. Identify A,B, C and D.

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Which of the following tools of recombinant DNA technology is incorrectly paired with its use?

What were the important discoveries that led to the stepping stones of recombinant DNA technology?

a. What are molecular scissors ? Give one example . b. Explain their role in recombinant DNA technology .

The different steps of recombinant DNA technology are given below randomly. (i) Isolation of the DNA fragments or genes to be cloned (ii) Introduction of the recombinant DNA into a suitable cell (usually E. coil) called host (transformation) (iii) Multiplication/expression of the introduced gene in the host (iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment (v) Insertion of the isolated gene in a suitable plasmid vector Which of the following represents the correct sequences of steps ?

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The process of recombinant DNA technology has the following steps I. Amplification of the gene. II. Insertion of recombinant DNA into the host cells. III. Cutting of DNA at specific location using restriction enzyme. IV.Isolation of genetic material orial (DNA)Pick out the correct sequence of step for recombinant DNA technology.