Write about the induced breeding method in fishes ?

Explanation

Induced breeding: The fish seed is commonly collected from breeding grounds but does not guarantee that all fish seeds belong to the same species Hence advanced techniques have been developed to improve the quality of fish seed by artificial method of fertilization and induced breeding. Artificial fertilization involves removal of ova and sperm from female and male by artificial mechanical process and the eggs are fertilized. For artificial fertilization the belly of mature female fish is held upward. Stripping is done with the thumb of the right hand from the anterior to posterior direction for the ejection of eggs due to force. In this way eggs are collected separately. Further, the male fish is caught with its belly downwards. The milt of fish is striped and collected separately, and then the eggs are fertilized.
Induced breeding is also done by hypophysation (removal of pituitary gland). The gonadotropin hormone (FSH and LH) secreted by the pituitary gland influences the maturation of gonads and spawning in fishes. Pituitary gland is removed from a healthy mature fish. Pituitary extract is prepared by homogenising in 0.3% saline or glycerine and centrifuged for 15 minutes at 8000 rpm. The supernatant is injected intramuscularly at the base of the caudal fin or intra-peritonealy at the base of pectoral fin. Male and female fishes start to spawn (release of gametes) and eggs are fertilized. The fertilized eggs are removed from the spawning place and kept into hatching hapas.