How will you avoid the growing of microbes in nutrient medium during culture process? What are the techniques used to remove the microbes?

Explanation

The culture medium is sterilized before tissue culture . The culture media are placed in glass containers and are plugged with non-absorbant cotton. Then it is autoclaved as 15 psi `(121^@C)` for 15 to 30 minutes. The other contents like vitamins,amino acid hormones and plant extracts are passed through millipore membrane with 0.2 mm pore diameter and then pored into the sterilized medium inside the laminar Airflow chamber under sterile condition. This procedure will eliminate microbes in the culture medium.