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Write the steps to sterilize the explant...

Write the steps to sterilize the explants in plant tissue culture?

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The tissue culture room sterilization starts with washing of floor and walls with detergent water and then wiped with 2% sodium hypochlorite or 95% ethanol. The cabinct of laminar air flow champer and the working space is wiped with 95% ethanol and subjected to uv radiation for 15 minutes, in order to get rid of all microbes in the culture room.
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