Would you like to choose an exonuclease enzyme while produing a recombinant DNA molecule ?

You have created a recombinant DNA molecules by ligating a gene to a plasmid vector. By mistake , your friend adds exonuclease enzyme to the tube containing the recombinant DNA. How will your experiment get affected as you plan to go tranformation now?

The role of DNA ligae in the construction of a recombinant DNA molecule is:

Name the enzyme that helps in forming recombinant DNA.

Write about any three enzymes needed for recombinant DNA technology.

Would you like to buy a house having windows but no ventilators?

Whatdo you understandby recombinant DNA technology?

A plasmid and a DNA sequence in a cell need to be cut for producing recombinant DNA. Name the enzyme which cts as a molecular scissors to cut the DNA segments.

Vectors with single or very few recognition sites for a particular restriction enzyme are preferred in recombinant DNA technology (RDT). Justify.