A plasmid DNA and a linear DNA (both of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using:

Two questions consist of two statements each, printed as Assertion and Reason. While answering these questions, you required to choose any one of the following four responses. If both Assertion and Reason are true and Reason is a correct explanation of Assertion. If both Assertion and Reason are true and Reasons is not a correct explanation of Assertion. If Assertion is true but Reason is false. If both Assertions and Reasons are false. Assertion:Three main types of restriction endonucleases i.e., Type I, Type II and Type III are known with slightly different mode of action. Reason:In palindromes with rotational symmetry second half of complementary strand in DNA double helix is the mirror image of base sequence in the first half of another strand.

Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.

These questions consist two statements each, printed as Assertion and Reason. While answering these questions you are required to choose any one of the following four responses. If both Assertion and Reason are true and Reason is correct explanation of Assertion. If both Assertion and Reason are true but Reason is not correct explanation of Assertion. If Assertion is true but Reason is false. If both Assertion and Reason are false. Assertion:DNA fingerprints involves identifying difference in some specific regions in DNA sequences. Reason: In repetitive DNA sequences, a small stretch of DNA in repeated many times.

Two questions consist of two statements each, printed as Assertion and Reason. While answering these questions, you required to choose any one of the following four responses. If both Assertion and Reason are true and Reason is a correct explanation of Association. If both Association and Reason are true and Reasons is not a correct explanation of Assertion. If Assertion is true but Reason is false. If both Assertions and Reason are false. Assertion: DNA segments can be excised by 'molecular scissors or chemical scalpels' what biotechnologists call restriction enzymes. Reason: Restriction enzymes are synthesized by microbes as a defence mechanism are specific endonucleases which are cleave double stranded DNA.

Two questions consist of two statements each, printed as Assertion and Reason. While answering these questions, you required to choose any one of the following four responses. If both Assertion and Reason are true and Reason is a correct explanation of Association. If both Association and Reason are true and Reasons is not a correct explanation of Assertion. If Assertion is true but Reason is false. If both Assertions and Reasongs are false. Assertion: Recognition site should be preferably single and responsive to commonly used restriction enzyme. Reason:In pBR 322 alien DNA is ligated generally in the area of Bam-HI site of tetracycline resistance gene.

Two questions consist of two statements each, printed as Assertion and Reason. While answering these questions, you required to choose any one of the following four responses. If both Assertion and Reason are true and Reason is a correct explanation of Association. If both Association and Reason are true and Reasons is not a correct explanation of Assertion. If Assertion is true but Reason is false. If both Assertions and Reasongs are false. Assertion:Genetic engineering is essentially the alteration of the genetic make up of cells by deliberate and artifical means Reason: It involves transfer or replacement of genes to create recombinant DNA.

Two questions consist of two statements each, printed as Assertion and Reason. While answering these questions, you required to choose any one of the following four responses. If both Assertion and Reason are true and Reason is a correct explanation of Association. If both Association and Reason are true and Reasons is not a correct explanation of Assertion. If Assertion is true but Reason is false. If both Assertions and Reasongs are false. Assertion:Genetic engineering is also called recombinant DNA technology. Reason: It brings about improvement of genetic make up of an organism.

A biotechnologist wanted to create a colony of E.coli possessing the plasmid pBR322, sensitive to Tetracycline. Which one of the following restriction sites would he use to ligate a foreign DNA?