Define restriction site.

Show with a sketch of E-coli cloning vector showing restriction sites.

List the features required to facilitate cloning into vector. Show with a sketch the E. coli cloning vector showing restriction site.

Redraw diagram of cloning vector pBR 322 given below and label the parts A, B , C, D showing restriction sites.

A biotechnologist wanted to create a colony of E.coli possessing the plasmid pBR322, sensitive to Tetracycline. Which one of the following restriction sites would he use to ligate a foreign DNA?

explain pBR 322 plasmid and label the following in it: Any two restriction sites.

Write the role of 'ori' and 'restriction' site in a cloning vector pBR 322.

Which of the following restriction enzymes produces blunt ends?

In the nomenclature of enzyme restriction endonuclease, the Roman numerals indicates:

Define recombinant DNA technology.What is the function of restriction enzymes?