explain pBR 322 plasmid and label the following in it:
Any two restriction sites.

In a sketch of pBR 322 plasmid and label the following in it: An antibiotic resistant gene.

Explain briefly the following Restriction enzymes

Redraw diagram of cloning vector pBR 322 given below and label the parts A, B , C, D showing restriction sites.

Label the following in pBR 322 plasmid Ori and rop genes.

Explain with two examples the following ligands.

A biotechnologist wanted to create a colony of E.coli possessing the plasmid pBR322, sensitive to Tetracycline. Which one of the following restriction sites would he use to ligate a foreign DNA?

Explain with two examples the following Coordination sphere.

Explain with two examples the following Central atom or ion.

Explain with two examples the following oxidation number of central atom.

ADDITIONAL QUESTIONS : The diagram represents a flower. Label the parts and answer the following : (a) Give the letter of the structure which : (i) becomes the fruit wall, (ii) becomes the testa (seed coat), (iii) produces pollen grains. (b) Explain two ways, shown in the diagram, in which this flower is adapted for insect pollination.