In a sketch of pBR 322 plasmid and label the following in it:
An antibiotic resistant gene.

explain pBR 322 plasmid and label the following in it: Any two restriction sites.

Label the following in pBR 322 plasmid Ori and rop genes.

Cohen and Boyer isolated an antibiotic resistance gene,by cutting out a piece of DNA from a plasmid which was responsible for conferring antibiotic resistance, in the year:

Explain the importance of (a) ori, (b) amp^R and (c ) rop in the E. coli vector shown below: ampR:Ampicillin antibiotic resistance gene. It is required for separation of recombinant DNA from non-recombinant. rop: The gene which forms proteins involved in replication of plasmid.

Redraw diagram of cloning vector pBR 322 given below and label the parts A, B , C, D showing restriction sites.

Two questions consist of two statements each, printed as Assertion and Reason. While answering these questions, you required to choose any one of the following four responses. If both Assertion and Reason are true and Reason is a correct explanation of Association. If both Association and Reason are true and Reasons is not a correct explanation of Assertion. If Assertion is true but Reason is false. If both Assertions and Reasongs are false. Assertion: Recognition site should be preferably single and responsive to commonly used restriction enzyme. Reason:In pBR 322 alien DNA is ligated generally in the area of Bam-HI site of tetracycline resistance gene.

In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (i-iv) in ths regard and select the correct option about which one or more of these can be used as vector/vectors: (i) Bacterium (ii) Plasmids (iii) Plasmodium (iv) Bacteriophage

These questions consist two statements each, printed as Assertion and Reason. While answering these questions you are required to choose any one of the following four responses. If both Assertion and Reason are true and Reason is correct explanation of Assertion. If both Assertion and Reason are true but Reason is not correct explanation of Assertion. If Assertion is true but Reason is false. If both Assertion and Reason are false. Assertion:'Bt' toxin gene has been cloned from bacteria and expressed in plants to provide resistance from insect wihtout in need of insecticides. Reason: 'Bt' toxin is produced from bacterium Bacillus thuringiensis.

A biotechnologist wanted to create a colony of E.coli possessing the plasmid pBR322, sensitive to Tetracycline. Which one of the following restriction sites would he use to ligate a foreign DNA?

Fill in the blanks the selectable marker in PBR 322 is .......... resistance gene.