To act as a vector DNA molecule should bear origin of replication (Ori). is it true?

You have created a recombinant DNA molecules by ligating a gene to a plasmid vector. By mistake , your friend adds exonuclease enzyme to the tube containing the recombinant DNA. How will your experiment get affected as you plan to go tranformation now?

During DNA replication, why is it that the entire molecule does not open in one go ? Explain replication fork.

Name the enzyme and state its property that is responsible for continuous and discountinuous replication of the two strands of a DNA molecules.

Write 'True' or 'False': Hind II always join DNA molecules at specific site by recognising a particular sequence of six base pairs.

Three particles, each of mass 'm' are situated at the vertices of an equilateral triangle of side 'a'. the only force acting on the particles are their mutual gravitatinoal foces. It is desired that each particle move on a circle, while maintaining the original mutual separation 'a'. Find the initial velocity that should be given to each particle and also the time period of the circular motion.

Draw the vector DNA and a foreign DNA showing the sites where EcoRI has acted to form the sticky ends.

A force F=2hat(i)+hat(j)-hat(k) acts at point A whose position vector is 2hat(i)-hat(j) . Find the moment of force F about the origin.

Read the following four statements (A-D) about certain mistakes in two of them: The first transgenic buffalo Rosie produced milk which was human alpha-lactalbumin enriched. Restriction enzymes are used in isolation of DNA from other macro-molecules Downstream processing is one of the steps of R-DNA technology. Disarmed pathogen vectors are also used in transfer of R-DNA into the host.

Two questions consist of two statements each, printed as Assertion and Reason. While answering these questions, you required to choose any one of the following four responses. If both Assertion and Reason are true and Reason is a correct explanation of Association. If both Association and Reason are true and Reasons is not a correct explanation of Assertion. If Assertion is true but Reason is false. If both Assertions and Reasongs are false. Assertion: Recognition site should be preferably single and responsive to commonly used restriction enzyme. Reason:In pBR 322 alien DNA is ligated generally in the area of Bam-HI site of tetracycline resistance gene.

Explain the importance of (a) ori, (b) amp^R and (c ) rop in the E. coli vector shown below: ampR:Ampicillin antibiotic resistance gene. It is required for separation of recombinant DNA from non-recombinant. rop: The gene which forms proteins involved in replication of plasmid.