Digrammatically represent the experimental steps in cloning and expressing an human gene [say the gene for growth hormone) into a bacterium like E. coli?

Write a short note on Production of human growth hormone by E.coli.

Steps involved in gene cloning are as follows: Complete the flow chart.

What is regulation of gene expression? Explain with the help of the lac operon of E.Coli.

Show the steps involved in the transfer of gene for production of human insulin.

Write 'True' or 'False': Stewart Linn and Werner Arber (1963) isolated two enzymes which restricted the growth of bacteriophage in bacterium E.coli.

These questions consist two statements each, printed as Assertion and Reason. While answering these questions you are required to choose any one of the following four responses. If both Assertion and Reason are true and Reason is correct explanation of Assertion. If both Assertion and Reason are true but Reason is not correct explanation of Assertion. If Assertion is true but Reason is false. If both Assertion and Reason are false. Assertion:'Bt' toxin gene has been cloned from bacteria and expressed in plants to provide resistance from insect wihtout in need of insecticides. Reason: 'Bt' toxin is produced from bacterium Bacillus thuringiensis.

What is an inducible operon ? Name the genes that constitute an operon. Explain the lac operon of E.Coli with help of schematic representation.

If a recombinant DNA bearing gene for ampicillin resistance is transferred into E. coli cells and the host cells are spread on agar plates containing amplicillin, then:

Explain the importance of (a) ori, (b) amp^R and (c ) rop in the E. coli vector shown below: ampR:Ampicillin antibiotic resistance gene. It is required for separation of recombinant DNA from non-recombinant. rop: The gene which forms proteins involved in replication of plasmid.