rDNA

Which one of the following is not the product of transgenic experiments ? A) Pest-resistant crop variety B) High nutritional value in grains C) Production of insulin by rDNA technique (D) Drought-resistant crops

With regard to insulin choose correct option. (a) C-peptide is not present in mature insulin (b) The insulin produced by rDNA technology had C-peptide (c)The pro-insulin has C-peptide (d) A-peptide and B-peptide of insulin are interconnected by disulphide bridges

Identify the desirable characteristics for a plasmid used in rDNA technology from the following (a) Ability to multiply and express outside the host in a bioreactor (b) A highly active promoter (c) A site at which replication can be initiated (d) One or more identifiable marker genes (e) One or more unique restriction sites

Arrange the following events in the correct order involved in prepartion of rDNA. I. Detergent lysis II. Elution . III. Restriction enzyme digestion IV Cell wall degradtion V. DNA spooling . VI. Gel electro phoresis . VII . Ligation with plasmid vector .

In bacterial culture some of the colonies produced blue colour in the presence of chromogenic substrate and some did not due to presence or absence of an inert (rDNA) in the coding sequence of B galactosidase. (a) Mention the mechanism and steps involved in above experiment. (b) How is it advantageous over simultaneous plating on two plates having different antibiotics.

Match the organism with its related contribution in the field of biotechnology. {:(,"Column-I",,"Column-II"),(1,"Escherichia coli",I,1^(st)rDNA),(2,"Agrobacterium tumefaciens",II,pBR322),(3,"Thermus aquaticus",III,"Ti plasmid"),(4,"Salmonella typhimurium",IV,"Polymerase used in PCR"):}

How is mature insulin different from proinsulin secreted humans pancreas in human? Explain how was human functional insulin produced using rDNA technology. Why is the functional insulim thus produced considered better than the ones used earlier by diabetic patients?

Read the following statements and select the incorrect ones. (i) When the transformed cells on agar plates containing ampicillin are spread, both transformed and untransformed cells with grow. (ii) Restriction enzymes are used in isolation and separation of DNA from other macromolecules. (iii) Downstream processing is one of the steps of rDNA technology. (iv) Disarmed pathogen vectors are also used in transfer of rDNA into the host.