The DNA replication is semi-conservative is proved by an experiment conducted by Meselson and Stahl in 1958. To perform their experiment they use heavy nitrogen (`""^(15)N`) in E. coli. The process of replication. in living cells requires a set of enzymes. The main enzyme is DNA dependent DNA polymerase. The DNA-A dependent DNA polymerase catalyse polymerization only in one direction, that is `5 to 3'`. In eukaryotes, the replication of DNA takes place at the S-phase of the cell cycle.
How many types of DNA polymerases associated with eukaryotic cell?

Options

A. Three
B. Six
C. Five
D. One

Explanation

To answer the question regarding the types of DNA polymerases associated with eukaryotic cells, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding DNA Replication**:
- DNA replication is a crucial process that occurs in cells, particularly during the S-phase of the cell cycle in eukaryotes. It is semi-conservative, meaning that each new DNA molecule consists of one old strand and one new strand.

2. **Role of DNA Polymerases**:
- DNA polymerases are enzymes that play a vital role in synthesizing new DNA strands by adding nucleotides to a growing chain. They can only add nucleotides in the 5' to 3' direction.

3. **Types of DNA Polymerases in Eukaryotes**:
- Eukaryotic cells contain several types of DNA polymerases, each with specific functions in DNA replication and repair. The main types of DNA polymerases found in eukaryotes are:
- **DNA Polymerase α (alpha)**: Initiates DNA synthesis.
- **DNA Polymerase β (beta)**: Involved in DNA repair.
- **DNA Polymerase γ (gamma)**: Replicates mitochondrial DNA.
- **DNA Polymerase δ (delta)**: Responsible for lagging strand synthesis.
- **DNA Polymerase ε (epsilon)**: Responsible for leading strand synthesis.

4. **Conclusion**:
- In total, there are **five main types of DNA polymerases** associated with eukaryotic cells: DNA polymerases α, β, γ, δ, and ε.

### Final Answer:
There are **5 types of DNA polymerases** associated with eukaryotic cells.

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