How did Eli Lilly Company go about preparing the human insulin? How is the insulin thus produced different from that produced by the functional human insulin gene?

How did Eli Lilly synthesise the human insulin? Mention one difference between this insulin and the one produced by the human pancreas.

Human insulin is being commercially produced from a transgenic species of:

Why is the functional insulin produced, considered better than the ones used earlier by diabetic patients?

How did an American Company, Eli Lilly use the knowledge of rDNA technology to produce human insulin?

A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reason could be (a) Human gene may have intron which bacteria cannot process (b) Amino acid codons for humans and bacteria are different (c) Human protein is formed but degraded by bacteria (d) all of the above

The biotech company, which commercially manufactured the first recombinant human insulin.

Which biotechnology company is credited with the synthesis of genetically engineered human insulin for the first time ?

Some of the steps involved in the production of humulin are given below. Choose the correct sequence. (i) Synthesis of gene (DNA for human insulin artificially. (ii) Culturing recombinant E.coli in bioreactors. (iii) Purification of humulin. iv Insertion of human insulin gene into plasmid (v) Introduction of recombinat plasmid into E.coli (vi) Extraction of recombinant gene product from E.coli.

Discuss in detail the preparation of benzene diazonium chloride from aniline. Why it is not separated in solid state and used as soon as produces in solution? How can you convert benzene diazonium chloride to fluoro and iodobenzene.