Inbreeding for five generations led to production of homozygous transgenic mice.However, these homozygous males or females were infertile. Which of the following approaches is most preferable and economical to obtain heterozygous transgenic animals continuously ?

Options

A. More transgenic founder (`1^(st)` animal) should be generated.
B. Crossing (breeding) of transgenic mice with wild typw mice in earlier generations should be done for continued production of transgenic heterozygous offsprings.
C. Inbreeding should be avoided after `5^(th)` generation.
D. Homozygous transgenic mice should be mated with heterozygous transgenic mice for continued production of transgenic heterozygous offsprings.

Answer

B

Explanation

Some of the transgenic mice should be bred with wild type mice of earlier generations to maintain a continuous supply of heterozygous transgenic mice by simple process of mating instead of involving complex biotechnological methods. Rest of the transgenic mice can be inbred for give generations to produce homozygous transgenic mice.