Which of the following statements is not correct regarding EcoRl restriction endonuclease enzyme ?

Options

A. It is isolaed from Escherichia coil `RY 13`
B. Its recongition sequence is `5' -GA A T T C-3'`
`3'-C T TA AG -5'`
C. It produces complementary blunt ends.
D. None of these

Answer

C

Explanation

The restriction endonuclease enzyme `EcoRl` was isolated from bacterium Escherichia coil `RY13`. It recognises the base sequence GA AT TC in DNA deplex and cuts its strand between G and A as shown below.
`5'overset(darr)-A-A-T-T-C-3'`
`3'-C-T-T-A-Aunderset(uarr)-G-5'`
It results in complementary sticky ends.