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If you want of recover many copies of th...

If you want of recover many copies of the target DNA, you will choose a vector

A

Which does not have origin of replication

B

which has antibiotic resistance gene

C

whose origin supports high copy number

D

which has only one restriction site

Text Solution

Verified by Experts

The correct Answer is:
C
Vectors that have high number per cell will have high copy number of their genome within the bacterial cell. If we link an alien piece of DNA with vectorm we can multiply its number equal to the copy number of the vector. Any piece of DNA when linked to the 'ori' sequence, can be made to replicate within the host cells. This property of 'ori' is used to make a number of copies of the linked DNA. If we want to obtain many copies of the target DNA, then it should be cloned in such a vector whose 'ori' supports high copy number.
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