Identify A,B,C, and D in the given figure of E. coli cloning vector pBR322 and select the correct option.

Options

A. `{:(A,B,C,D),("Hindl",EcoRl,amp^(R),"ori"):}`
B. `{:(A,B,C,D),("Hindl",BamHl,Kan^(R),amp^(R)):}`
C. `{:(A,B,C,D),(BamHl,Pstl,"ori",amp^(R)):}`
D. `{:(A,B,C,D),(EcoRl,BamHl,amp^(R),"ori"):}`

Answer

D

Explanation

`pBr322` was the first artificial cloning vector constructed in 1977 by Boliver and Rodriguez. It is widely used in gene cloning eperiments. In `pBR322` plasmid vector, p-denotes that it is a plasmid, `BR-` stands for Boliver and Rodriguez who constructed this plasmid, 322 - Is a number given to distinguish this plamid from others developed in the same laboratory. coil cloning vector `pBR 322` has restriction sites (Hindll, `EcoRl, BamHl, Sall, Pvull, Pstll, Clal)`, ori (origin of replication) and antibiotic resistance genes `(amp^(R)` and `tet^(R))`. rop codes for the proteins involved in the replication of the plasmid.