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In pBR322, tetracycline resistance gene ...

In pBR322, tetracycline resistance gene `(tet^(R))` has recognition site for which of the following restriction endonuclease ?
1) Hindlll
2) BamHl
3) EcoRl
4) Pstl

A

1) Hindlll

B

2) BamHl

C

3) EcoRl

D

4) Pstl

Text Solution

Verified by Experts

The correct Answer is:
B
In plamid vector `pBR322`, two unique restriction sites Pstl and Pvul are located within the `amp^(R)` gene and BamHl, Sall, etc, are located within the `tet^(R)` gene. The presence of restriction sites within the marker genes `tet^(R)` and`amp^(R)` permits and easy selection for cells transformed with the recombinant `pBR322`. When restriction enzyme BamHl or Sal l is used, the DNA insert is placed within the gene `te^(R)` making it nonfunctional.
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