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In the process of insertional inactivati...

In the process of insertional inactivation
1) a recombinant DNA is inserted within the coding sequence of enzyme β -galactosidase, resulting in inactivation of the enzyme
2) a recombinant DNA is inserted within the coding sequence of proteins involved in the replication of the plasmid
3) a recombinat DNA is inserted within the recongnition site for EcoRl
4) none of these

A

1) a recombinant DNA is inserted within the coding sequence of enzyme `beta`-galactosidase, resulting in inactivation of the enzyme

B

2) a recombinant DNA is inserted within the coding sequence of proteins involved in the replication of the plasmid

C

3) a recombinat DNA is inserted within the recongnition site for EcoRl

D

4) none of these

Text Solution

Verified by Experts

The correct Answer is:
A
Alternative selectable markers have been developed which differentiate recombinants from the non-recombinants on the basis of their ability to produce colour in the presence of a chromogenic substrate. In this, a recombinantDNA is inserted within the coding sequence of an enzyme, `beta`-galactosidase. This results into inactivation of the enzyme, whcih is referred to as insertional inactivation. The presence of a chromogenic substrate given blue coloured colonies if the plasmid in the bacteria does not have an insert. Presence of insert results into insertional inactivation of the `beta`-galactosidase and the colonies do not produce any colour, these are identified as recombinant colonies.
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