Home
Class 12
BIOLOGY
During insertional inactivation, the pre...

During insertional inactivation, the presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. The blue colour is produced by the enzyme
1) α -glucosidase
2) restriction endonuclease
3) β -galactosidase
4) Taq polymerase

A

1) `alpha`-glucosidase

B

2) restriction endonuclease

C

3) `beta`-galactosidase

D

4) Taq polymerase

Text Solution

Verified by Experts

The correct Answer is:
C
Promotional Banner

Similar Questions

Explore conceptually related problems

Read the given statements and select the correct option Statement 1 : In insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome. Statement 2: Presence of insert results into insertional inactivation of beta -galactosidase enzyme and the colonies do not produce any colour. A) Both statements 1 and 2 are correct B) statement 1 is correct but statement 2 is incorrect C) statement 1 is incorrect but statement 2 is correct D) Both statements 1 and 2 are incorrect

In the process of insertional inactivation 1) a recombinant DNA is inserted within the coding sequence of enzyme β -galactosidase, resulting in inactivation of the enzyme 2) a recombinant DNA is inserted within the coding sequence of proteins involved in the replication of the plasmid 3) a recombinat DNA is inserted within the recongnition site for EcoRl 4) none of these

An organic compound A having molecular formula C_(2)H_(5)O_(2)N on treatment with HNO_(2) gives a blue coloured compound B having molecular formula C_(2)H_(4)O_(3)N_(2) . On reduction, A gives a compound C having molecular formula C_(2)H_(7)N . C on treatment with nitrous acid gives D which gives positive iodoform test. Identify the compounds A, B, C and D.