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The correct sequence of making a cell co...

The correct sequence of making a cell competent is

A

1. treatment with divalent cation `rarr` incubation of cells with recombinant `DNA` of ice `rarr` heat shock `(42^(@)C)rarr` placing on ice

B

2. heat shock `(42^(@)C)rarr` incubation of cells with recombinant DNA on ice `rarr` treatment with divalent cations `rarr` placing on ice

C

3. treatment with divalent cation `rarr` placing on ice `rarr` incubation of cells with recombinant DNA on ice `rarr` heat shock `(42^(@)C)`

D

4. incubation of cells with recombinant DNA on ice `rarr` heat shock `(42^(@)C) rarr `treatment with divalent cations `rarr` placing on ice.

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The correct Answer is:
A
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