Home
Class 12
BIOLOGY
Primers are 1) chemically synthesised ...

Primers are
1) chemically synthesised oligonucleotides that are complementary to the regions of DNA
2) chemically synthesised oligonucleotides that are not complementary to the regions of DNA
3) chemically synthesised, autonomously replicating circular DNA molecules
4) specific sequences present on recombinant DNA

A

1) chemically synthesised oligonucleotides that are complementary to the regions of DNA

B

2) chemically synthesised oligonucleotides that are not complementary to the regions of DNA

C

3) chemically synthesised, autonomously replicating circular DNA molecules

D

4) specific sequences present on recombinant DNA

Text Solution

Verified by Experts

The correct Answer is:
A
Primers are small, chemically synthesised oligonucleotides that are complementary to the sequences present at 3' end of the template DNA. They hybridise to the target DNA region, one to each strand of the double helix. These primers are oriented with their ends facing each other allowing synthesis of the DNA towards one another.
Promotional Banner

Similar Questions

Explore conceptually related problems

Rearrange the following in the correct sequence to accomplish an important biotechnological reaction (a) in vitro synthesis of copies of DNA of interest (b) Chemically synthesised oligonucleotides (c) Enzyme DNA polymerase (d) Complementary region of DNA (e) Genomic DNA template (f) Nucleotides provided (g) Primers (h) Thermostable DNA-polymers (From Thermus aquaticus) (i) Denaturation of dsDNA

Depending upon the chemical nature of the template (DNA or RNA) and the nature of nucleic acids synthesised from it (DNA or RNA), list the types of nucleic acid polymerases.

In a double standed DNA, the sequence of nucleotides in one strand is 3' ATTCGCTAT 5'. What will be the complementary squence on the other strand ?

Assertion : A piece of DNA inserted into an alien organism generally does not replicate if not inserted into a chromosome. Reason : Chromosomes have specific sequences called ori region where DNA replication is initiated.

The base sequence in one of the strands of DNA is TAGCATGAT. (i) Give the base sequence of the complementary strand. (ii) How are these base pairs held together in a DNA molecule? (iii) Explain the base complementarity rule. Give the name of the scientist who framed this rule?

Read the given statements and select the correct option. Statement 1 : Chloroplast and mitochondria are semiautonomous bondies. Statement 2 : Chloroplast and mitochondria have their own DNA and protein synthesising machinery.