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Enzyme 'Taq polymerase' used in PCR, has...

Enzyme 'Taq polymerase' used in PCR, has been isolated from bacterium
1) Agrobacterium tumefaciens
2)Thermus aquaticus
3) Streptomyces albus
4) Escherichia coil

A

1) Agrobacterium tumefaciens

B

2)Thermus aquaticus

C

3) Streptomyces albus

D

4) Escherichia coil

Text Solution

Verified by Experts

The correct Answer is:
B
The final step of `PCR` is extension, wherein `Taq DNA` polymerase (isolated from a thermophilic bacterium Thermus aquaticus) synthesies the DNA region between the primers, using `DNTPs` (denoxynucleoside triphosphates) and `Mg^(2+)`. The primers are extened towards each other so that the DNA segment lying between the two primers is copied. The optimum temperature for this polymerisation step is `72^(@)C`. Taq polymerase remains active during high temperature induced denaturation of double stranded DNA.
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