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The correct sequence of different steps ...

The correct sequence of different steps of polymerease chain reaction is
1) annealing → denaturation → extension
2) denaturation → extension → annealing
3) denaturation → annealing → extension
4) extension → denaturation → annealing

A

1) annealing `rarr` denaturation `rarr` extension

B

2) denaturation `rarr` extension `rarr` annealing

C

3) denaturation `rarr` annealing `rarr` extension

D

4) extension `rarr` denaturation `rarr` annealing

Text Solution

Verified by Experts

The correct Answer is:
C
Polymerase chain reaction is a technique used to replicate a fragment of DNA so as to produce many copies of a particular DNA sequence. A single PCR amplification cycle involves three basic steps : denaturation, annealing and extension (polymerisation).
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