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The role of DNA ligase in the constructi...

The role of DNA ligase in the construction of a recmobinant DNA molecule is

A

a) formation of phosphodiester bond between two DNA fragments

B

b)formation of hydrogen bonds between sticky ends of DNA fragments

C

c) ligation of all purine and pyrimidine bases

D

d)none of the above

Text Solution

Verified by Experts

The correct Answer is:
A
DNA ligases (joinin or sealing enzymes) are also called genetic gum. They join two individual fragments of double stranded DNA by forming phosphodiester bonds between them. Thus, they help in sealing gaps in DNA fragments. Therefore, they act as a molecular glue. The enzyme used most often in the `rDNA` technology is `T_(4)DNA` ligases.
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In the DNA molecule:

The given flow chart depicts the steps to transfer a desirable gene of interest into a plant. Identify the missing steps (A,B and C) with regard ot following statements and select the correct option. (i) Joining of desirable gene to a suitable cloning vector using ligases to create a recombinant DNA molecule. (ii) Selection of transformed cells. (iii) Transferring the recombinant DNA molecules to teh target cells.

Assertion : Genetic engineering requires both nuclease and ligases. Reason : Ligases produce the nick in the recombinant DNA molecule.

DNA ligase is commonly known as

The sticky ends of a fragmented DNA molecule are made of