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Which of the following steps are catalys...

Which of the following steps are catalysed by Taq polymerase in a PCR reaction ?
(a) Denaturation of template DNA
(b) Annealing of primers to template DNA
(c) Extension of primer and on the template DNA
(d) All of the above

A

Denaturation of template DNA

B

Annealing of primers to template DNA

C

Extension of primer and on the template DNA

D

All of the above

Text Solution

Verified by Experts

The correct Answer is:
C
The final step of PCR is extension, wherein Taq DNA polymerase (isolated from a thermophilic bacterium Thermus aquaticus) synthesises the DNA region between the primers, using dNTPs (denoxynucleoside triphosphates) and `Mg^(2+)`. The primers are extended towards each other so that the optimum temperature for this polymerisation step is `72^(@)C`. Taq polymerase remains active during high temperature induced denaturation of double stranded DNA.
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