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Assertion : Special methods are used for...

Assertion : Special methods are used for transformation i.e., incorporation of recombinant DNA into host.
Reason : DNA is a hydrophilic molecule.

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Transformation is permanent heritable change in a cell, particularly a bacterial cell, that occurs as a result of its acquiring foreign DNA. But cells normally do not take up foreign DNA molecule because DNA is a hydrophilic macromolecule and its movement through lipid membrane is opposed. special methods such as `CaCI_(2)` treatment, electroporation, particle delivary method, etc, are used to make host cells competent i.e., able to take up foreign DNA.
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