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Assertion : Use of chitinase enzyme is n...

Assertion : Use of chitinase enzyme is necessary for isolation of DNA from yeast cells but not in case of Spirogyra.
Reason : Fungal cell wall is made up of fungal cellulose or chitin.

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The correct Answer is:
A
Release of DNA from a cell is achieved by treating the cells or tissues with enzymes such as lysozyme (bacterial), cellulose (plant cells), chitinase (fungus), etc. These enzymes degrade cell wall, plasma membrane degrading enzymes like lipase, etc. are also needed. Since yeast is a fungus and fungal cell with is made of chitin (fungal cellulose), isolation of DNA necessarily requires use of enzyme chitinase.
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