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Restriction' in restriction enzyme refer...

Restriction' in restriction enzyme refers to

A

cleaving of phosphodiester bond in DNA by the enzyme.

B

cutting of DNA at specific position only

C

prevention of the multiplication of bacteriophage in bacteria.

D

All of the above

Text Solution

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The correct Answer is:
B

Restriction enzymes (also called molecular scissors) are responsible for cutting DNA. These enzymes belong to a class of enzymes called nucleases and are of two types : (i) Exonuclease which cut DNA at the ends and (ii) endonucleases which make cuts at specific positions within the DNA. The term .restriction. refers to the function of these enzymes in restricting the propagation of foreign DNA of bacteriophage in host bacterium, that is, cutting of DNA, at specific position only.
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