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Which of the following has popularised t...

Which of the following has popularised the PCR (polymerase chain reactions)?

A

Easy availability of DNA template

B

Availability of synthetic primers

C

Availability of cheap deoxyribonucleotides

D

Availability of "Thermostable' DNA polymerase

Text Solution

Verified by Experts

The correct Answer is:
D
The polymerase chain reaction (PCR) is a reaction in which amplification of specific DNA sequences is carried out in vitro Such repeated amplification is achieved by the using thermostable DNA polymerase (isolated from a bacterium, Thermus aquaticus) enzyme which remains active and stable during high temperature and induced denaturation of double-standard DNA.
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