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Assertion (A): It is essential to have f...

Assertion (A): It is essential to have few cloning sites in cloning vector. Reason(R): It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.

A

Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).

B

Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).

C

Assertion (A) is true but reason (R) is false.

D

Assertion (A) is false but reason (R) is true.

Text Solution

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The correct Answer is:
### Step-by-Step Text Solution: 1. **Understanding the Assertion (A)**: The assertion states that it is essential to have few cloning sites in a cloning vector. Cloning vectors are tools used in biotechnology to transfer genetic material into a host cell. The term "few cloning sites" typically refers to the presence of multiple cloning sites (MCS) that allow for the insertion of DNA fragments. 2. **Understanding the Reason (R)**: The reason provided states that having few cloning sites helps in identifying and eliminating non-transformants while selectively permitting the growth of transformants. Transformants are cells that have successfully incorporated the foreign DNA, while non-transformants have not. 3. **Role of Cloning Vectors**: Cloning vectors, such as plasmids, are designed to carry foreign DNA into a host cell. They contain features such as: - **Multiple Cloning Sites (MCS)**: These are regions containing several restriction enzyme sites that allow for the insertion of DNA. - **Selectable Markers**: These are genes that confer resistance to antibiotics or other substances, enabling the identification of cells that have taken up the vector. 4. **Importance of Selectable Markers**: Selectable markers are crucial as they help distinguish between transformants and non-transformants. Only the cells that have successfully taken up the vector (transformants) will survive in the presence of the antibiotic, while non-transformants will not. 5. **Evaluating the Assertion and Reason**: - Both the assertion and reason are true statements. - However, the reason does not correctly explain the assertion. The assertion focuses on the necessity of having multiple cloning sites for DNA insertion, whereas the reason discusses the role of selectable markers in identifying transformants. 6. **Conclusion**: Therefore, the correct evaluation is that both assertion and reason are true, but the reason is not the correct explanation for the assertion. ### Final Answer: Both assertion and reason are true, but the reason is not the correct explanation for the assertion. ---
### Step-by-Step Text Solution: 1. **Understanding the Assertion (A)**: The assertion states that it is essential to have few cloning sites in a cloning vector. Cloning vectors are tools used in biotechnology to transfer genetic material into a host cell. The term "few cloning sites" typically refers to the presence of multiple cloning sites (MCS) that allow for the insertion of DNA fragments. 2. **Understanding the Reason (R)**: The reason provided states that having few cloning sites helps in identifying and eliminating non-transformants while selectively permitting the growth of transformants. Transformants are cells that have successfully incorporated the foreign DNA, while non-transformants have not. 3. **Role of Cloning Vectors**: Cloning vectors, such as plasmids, are designed to carry foreign DNA into a host cell. They contain features such as: - **Multiple Cloning Sites (MCS)**: These are regions containing several restriction enzyme sites that allow for the insertion of DNA. ...
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