A schematic representation of polymerase chain reaction (PCR) up to the extension stage is given below.

Which of the following has popularized the PCR (Polymerase Chain Reaction) ?
a) Easy availability of DNA template
b) Availability of synthetic primers
c) Availability of cheap deoxyribonucleotides
d) Availability of thermostable DNA polymerase.

Options

A. Easy availability of DNA template
B. Availability of synthetic primers
C. Availability of cheap deoxyribonucleotides
D. Availability of thermostable DNA polymerase.

Answer

D

Explanation

Tag polymerase enzyme help to maintain the stability of DNA to be used repeatedly at high temperature in the PCR.