Which of the following is not a characte...

Which of the following is not a characteristic of pBR322 vector ?

A

1) It was the first artificial cloning vector constructed in 1977 by Boli- ver and Rodriguez.

B

2) It is the most widely used,versatile and easily manipulated vector.

C

3) It has two antibiotic resistance genes `tet^(R)` and `amp^(R)`

D

4) It does not have restriction site for sall.

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The correct Answer is:

D

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