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What will be the effect if pBR322, a clo...

What will be the effect if pBR322, a cloning vector does not carry 'ori' site ?

A

1) Sticky ends will not produce

B

2)Transformation will not take place

C

3) The cell will transform into a tumour cell

D

4) Replication will not take place

Text Solution

Verified by Experts

The correct Answer is:
D
For the multiplication of any alien piece of `DN` in an organism, it needs to be a part of a chromosome which has a specific sequence known as 'origin of replication' (ori). If ori is not present in a cloning vectorm replication will not be initiated.
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