Home
Class 12
BIOLOGY
Enzyme 'Taq polymerase' used in PCR, has...

Enzyme 'Taq polymerase' used in PCR, has been isolated from bacterium
1) Agrobacterium tumefaciens
2)Thermus aquaticus
3) Streptomyces albus
4) Escherichia coil

A

1) Agrobacterium tumefaciens

B

2)Thermus aquaticus

C

3) Streptomyces albus

D

4) Escherichia coil

Text Solution

Verified by Experts

The correct Answer is:
B
The final step of `PCR` is extension, wherein `Taq DNA` polymerase (isolated from a thermophilic bacterium Thermus aquaticus) synthesies the DNA region between the primers, using `DNTPs` (denoxynucleoside triphosphates) and `Mg^(2+)`. The primers are extened towards each other so that the DNA segment lying between the two primers is copied. The optimum temperature for this polymerisation step is `72^(@)C`. Taq polymerase remains active during high temperature induced denaturation of double stranded DNA.
Promotional Banner

Similar Questions

Explore conceptually related problems

Which of the following bacteria is used as a vector for plant genetic engineering ? 1) Agrobacterium tumefaciens 2) Bacteriophages 3) Thermus aquaticus 4) Pyrococcus furiosus

Identify the correct statements from the below I. Thermus aquatics is a bacterium which can tolerate high temperatures. (II) The first DNA polymerase enzyme was isolated from thermus aquatics. (III) T. aquatics used in polymerase chain reaction (PCR) for DNA amplification. (IV) Cyanobacteria are photosynthetic blue green algae.

The polymerase chain reaction is a technique used for 1) amplification of DNA 2) amplification of enzymes 3) amplification of proteins 4) all of these

_________a crown gall bacterium, is called an natural genetic engineer' of plants. 1) Escherichia coli 2) Streptomyces albus 3) Agrobacterium tumefaciens 4) Azotobacter

During insertional inactivation, the presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. The blue colour is produced by the enzyme 1) α -glucosidase 2) restriction endonuclease 3) β -galactosidase 4) Taq polymerase

Let I be the purchase value of an equipment and V(t) be the value after it has been used for t years. The value V(t) depreciates at a rate given by differential equation (d V(t)/(dt)=-k(T-t) , where k"">""0 is a constant and T is the total life in years of the equipment. Then the scrap value V(T) of the equipment is : (1) T^2-1/k (2) I-(k T^2)/2 (3) I-(k(T-t)^2)/2 (4) e^(-k T)

Across:1.fibre that is used as synthetic wool.2.Aplastic used for making water bottels.3.Along chain made of smallrepeating monomers.4. Another name for this semi-synthetic fibre is artificial silk.Down: 5. Atype of fibre that is naturally obtained from is cocoon.6.Asynthetic fibre classified as polyster. 7. A polymer used for making rope.

A potentiometer circuit has been set up for finding the internal resistance of a given cell.The main battery, used across the potentiometer wire, has an emf of 2.0 V and a negligible internal resistance.The potentiomter were itself is 4m long.When the resistance, R, connected across the given cell, has values of (i) Infinity (ii)9.5 Omega the'balancing lengths', on the potentiometerwire are found to be 3m and 2.85m, respectively. The value of internal resistance of cell is

In an objective paper, there are two sections of 10 questions each.For "section 1", each question has 5 options and only one optionis correct and "section 2" has 4 options with multiple answers and marks for a question in this section is awarded only if he ticks all correct answers. Marks for each question in "sectionl 1" is 1 and in "section 2" is 3. (There is no negative marking.) If a candidate attempts only two questions by guessing, one from "section 1" and one from "section 2", the probability that he scores in both question is