An antibiotic resistant gene in a vector usually helps in the selection of

Options

A. a) competent cells
B. b) transformed cells
C. c) recombinant cells
D. d)none of the above

Answer

B

Explanation

The ligation of aline DNA is carried out at a restriction site present in one of the two antibiotic resistance genes. For example, you can ligate a foreign DNA at the BamHl site of tetracycline resistance gene in the vectot pBR322. The recombinant plasmids will lose recombinants will grow in ampilcillin containing medium but not on that containing tetracycline, both the antibiotics. in this case, one antibiotic resistance gene helps in selecting the trasnformations, wherease the other antibiotic resistance gene gets inactivated due to insertion of alien DNA, and helps in selection of recombinants.