Which of the following statements are true regarding DNA polymerase used in PCR ?

Options

A. Denaturation of template DNA
B. Annealing of primers to template DNA
C. Extension of primer and on the template DNA
D. All of the above

Answer

C

Explanation

The final step of PCR is extension, wherein Taq DNA polymerase (isolated from a thermophilic bacterium Thermus aquaticus) synthesises the DNA region between the primers, using dNTPs (denoxynucleoside triphosphates) and `Mg^(2+)`. The primers are extended towards each other so that the optimum temperature for this polymerisation step is `72^(@)C`. Taq polymerase remains active during high temperature induced denaturation of double stranded DNA.