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Assertion : E.coli having pBR322 with DN...

Assertion : E.coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracyline.
Reason : Recoginition site for BamHI is present in `ter^(R)` region of pBR22.

A

If both assertion and reason are true and reason is the correct explanation of assertion

B

If both assertion and reason are true but reason is not the correct explanation of assertion

C

if assertion is true but reason is false

D

if both assertion and reason are false

Text Solution

Verified by Experts

The correct Answer is:
A
pBR322 has recognition sites for several commonly used restriction enzymes. Recognition site for BamHl is present in `tet^(r)` region i.e., region responsible for tetracyline resistance. When an insert is added at the BamHl recongnition site the gene for tetracycline resistance becomes non-functional and the recombinant bacteria with plasmid `pBR322` that has `DNA` insert at BamHl lose tetracycline resistance.
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