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Oswald Avery, Colin MacLeod and Maclyn M...

Oswald Avery, Colin MacLeod and Maclyn McCarty used enzymes to purify biochemicals such as proteins, DNA and RNA from the heat-killed S cells to see which ones could transform live R cells into S cells in Griffith's experiment. They observed that

A

Proteases and RNases affected transformation.

B

DNase inhibited transformation.

C

Proteases and Lipases affected transformation.

D

RNases inhibited transformation.

Text Solution

Verified by Experts

The correct Answer is:
B
Oswald Avery. Colin MacLeod and Maclyn McCarty found that DNA alone from S bacteria was able to transform R strain bacteria. Another observation was that the protein- digesting enzymes (proteases) and RNA- digesting enzymes (RNases) did not affect the process of transformation. This confirms that the transforming substance was not a protein or RNA.
Transformation was inhibited when digestion with DNase (DNA-digesting enzyme) was carried out, which proves that the DNA caused the transformation. Thus, they concluded that the DNA is the hereditary material but not all biologists were convinced.
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