Arrange the following steps in correct order: (TRANSCRIPTION):
(I) The nascent RNA separates and RNA polymerase also detaches and thus terminates the process of transcription.
(II)RNA polymerase loses the factor, but continues to add ribonucleotides to form RNA.
(III) Binding of RNA polymerase to the promoter and its association with initiation factor `(sigma)`.
(IV) The RNA polymerase associates with termination-factor `(rho)` transiently to stop the transcription process.

Options

A. (I) ,(II), (III), (IV)
B. (IV),(III),(II),(I)
C. (III),(II),(I),(IV)
D. (II),(III),(IV),(I)

Explanation

To arrange the steps of transcription in the correct order, we can follow the logical sequence of events that occur during the transcription process. Here’s the step-by-step solution:

### Step-by-Step Solution:

1. **Step III**: Binding of RNA polymerase to the promoter and its association with initiation factor (sigma).
- This is the initial step where RNA polymerase binds to the promoter region of the DNA, facilitated by the sigma factor, which is essential for the initiation of transcription.

2. **Step II**: RNA polymerase loses the factor, but continues to add ribonucleotides to form RNA.
- After the initial binding, the sigma factor is released, but RNA polymerase continues the elongation process by adding ribonucleotides to synthesize the RNA strand.

3. **Step I**: The nascent RNA separates and RNA polymerase also detaches, thus terminating the process of transcription.
- Once the RNA strand is synthesized, it separates from the DNA template, and RNA polymerase detaches from the DNA, marking the end of transcription.

4. **Step IV**: The RNA polymerase associates with termination factor (rho) transiently to stop the transcription process.
- In this final step, the rho factor helps in the termination of transcription, ensuring that the process is completed properly.

### Final Order:
- III → II → I → IV

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