Haploid plant cultures are obtained from

Options

A. leaves
B. root tip
C. pollen grain
D. buds

Answer

To answer the question "Haploid plant cultures are obtained from," we can follow these steps:

### Step-by-Step Solution:

1. **Understanding Plant Cultures**:
- Plant cultures involve taking a part of a plant and growing it in a nutrient medium under controlled conditions (in vitro). This can be any part of the plant, such as leaves, roots, or buds.

**Hint**: Remember that plant cultures can be derived from various parts of the plant, but the focus is on the type of cells they contain.

2. **Defining Haploid Plant Cultures**:
- Haploid plant cultures specifically refer to cultures that contain haploid cells, which have only one set of chromosomes. In plants, haploid cells are typically represented by gametes.

**Hint**: Think about what haploid means in the context of plant biology—it's all about the number of chromosome sets.

3. **Identifying the Source of Haploid Cells**:
- In plants, the male gametes are found in pollen grains. Pollen grains are produced in the anthers of flowers, and they are haploid.

**Hint**: Consider which part of the plant produces gametes and what type of cells they are.

4. **Pollen Culture Technique**:
- The technique used to obtain haploid plants from pollen grains is called pollen culture. This involves collecting pollen from the anther, sterilizing it, and then culturing it in a nutrient medium.

**Hint**: Recall the specific process of how pollen is harvested and cultured.

5. **Development of Haploid Plants**:
- When cultured, pollen grains can develop into a callus (an undifferentiated mass of cells). By treating this callus with plant hormones like auxin and cytokinin, we can promote rooting and differentiation, leading to the development of a complete haploid plant.

**Hint**: Remember the role of hormones in plant development and how they influence the growth process.

6. **Conclusion**:
- Based on the above information, haploid plant cultures are specifically obtained from pollen grains, as they are the only part of the plant that contains haploid cells.

**Final Answer**: Haploid plant cultures are obtained from **pollen grains**.